0986 4d

The d-psicose 3-epimerase (DPE) gene from Ruminococcus sp. kakek petir 4d slot was cloned and overexpressed in Escherichia coli. kakek petir 4d slot The recombinant protein was purified and characterized It was optimally active at pH 7 5–8 0 and 60 °C Activity was not dependent on the presence of metal ions; however, it became more thermostable with added Mn2+ The K m of the enzyme for d-psicose (48 mM) was lower than that for